What is rivularia?



This form is readily found on the underside of the leaves of water-lilies (Nuphar, Nymphaea, etc.), but is also abundant on submerged leaves and stems of other plants. It occurs in the form of translucent, gelatinous nodules of various sizes. Chromo-acetic acid gives beautiful preparations, but good results can also be secured from formalin or picric-acid material.

The most instructive preparations for morphological study can be obtained by the Venetian turpentine method. Stain in iron" haematoxylin and very lightly in erythrosin, the latter stain being used merely to outline the sheath. When ready for mounting, crush a small nodule under a cover-glass. The paraffin method is easily applied, since the gelatinous matrix keeps the filaments in place. Any form of similar habit may be prepared in the same way.





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